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5LKH

Cryo-EM structure of the Tc toxin TcdA1 in its pore state (obtained by flexible fitting)

EM specimen
VitrificationYes
InstrumentGATAN CRYOPLUNGE 3
EM Data Collection
MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
DetectorFEI FALCON II (4k x 4k)
3D Reconstruction
MethodSINGLE PARTICLE
Resolution (Å)3.46
Number of Particle30061
SoftwareRELION,EPU,CTFFIND,iMODFIT,DireX
SymmetryC5
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION

222415

PDB entries from 2024-07-10

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