5LKH
Cryo-EM structure of the Tc toxin TcdA1 in its pore state (obtained by flexible fitting)
EM specimen
Vitrification | Yes |
Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.46 |
Number of Particle | 30061 |
Software | RELION,EPU,CTFFIND,iMODFIT,DireX |
Symmetry | C5 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |