5LK1

Structure of hantavirus envelope glycoprotein Gc in postfusion conformation in presence of 200 mM KCL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976251
Spacegroup name	H 3
Unit cell lengths	107.289, 107.289, 127.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.550 - 1.700
R-factor	0.1786
Rwork	0.177
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz
RMSD bond length	0.011
RMSD bond angle	1.287
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.550	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.061	0.217
Number of reflections	56962
<I/σ(I)>	9.3	3
Completeness [%]	94.5	91.1
Redundancy	2.3	2.2
CC(1/2)	0.994	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES 6.5, 10.77%(v/v) PEG 8000, 7% (v/v) glycerol, 200 mM KCl