5LJY
Structure of hantavirus envelope glycoprotein Gc in complex with scFv A5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 116.623, 148.795, 37.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.268 - 3.000 |
| R-factor | 0.2165 |
| Rwork | 0.212 |
| R-free | 0.26980 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1471) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.760 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.121 | 0.922 |
| Number of reflections | 13990 | |
| <I/σ(I)> | 14 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.6 |
| CC(1/2) | 0.998 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 60 mM Na-HEPES 7.5, 40 mM hexamine cobalt chloride salt, 13.45% (w/v) PEG 4K, 7.4% (v/v) 2-propanol, and 1% (v/v) glycerol |
