5LJW
MamK non-polymerising A278D mutant bound to AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.572, 71.803, 79.761 |
| Unit cell angles | 90.00, 98.41, 90.00 |
Refinement procedure
| Resolution | 44.858 - 1.800 |
| R-factor | 0.1795 |
| Rwork | 0.178 |
| R-free | 0.20640 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.406 |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (dev_1810) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.083 | 0.499 |
| Number of reflections | 138054 | |
| <I/σ(I)> | 6.9 | 1.8 |
| Completeness [%] | 95.1 | 97.4 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.994 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 50 mM HEPES pH 7.3, 48 % (w/v) PEG 1000 |
