5LJB
Crystal structure of holo human CRBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91881 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.120, 49.142, 75.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.901 - 1.263 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.650 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.230 | 1.330 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.038 | 0.475 |
| Number of reflections | 34582 | |
| <I/σ(I)> | 17.5 | 2.9 |
| Completeness [%] | 99.0 | 94.5 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 279 | .2 M ammonium chloride, 0.1 M sodium acetate, 0.2 mM zinc chloride, pH 5,0, 20% PEG 6000 |
