5LJ8
Structure of the E. coli MacB periplasmic domain (P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.110, 54.860, 66.890 |
| Unit cell angles | 90.00, 92.86, 90.00 |
Refinement procedure
| Resolution | 32.870 - 1.950 |
| R-factor | 0.18043 |
| Rwork | 0.178 |
| R-free | 0.22772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftj |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.659 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.810 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 25260 |
| <I/σ(I)> | 8.1 |
| Completeness [%] | 99.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 30% Pentaerythritol ethoxylate (15/4 EO/OH), 6% polyvinylpyrrolidone, 100mM HEPES pH 7.5 |
