5LIS
Insulin solved by Native SAD from a dataset collected in one second
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.55 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 76.870, 76.870, 76.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.440 - 2.293 |
| R-factor | 0.22149 |
| Rwork | 0.217 |
| R-free | 0.26186 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.440 | |
| High resolution limit [Å] | 2.293 | 2.293 |
| Rmeas | 0.027 | |
| Number of reflections | 5420 | |
| <I/σ(I)> | 31.14 | |
| Completeness [%] | 85.0 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% ethylene glycol |
