5LID
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromopromazine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91983 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.720, 267.910, 111.330 |
Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
Resolution | 49.537 - 4.500 |
R-factor | 0.2148 |
Rwork | 0.212 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.219 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.540 |
High resolution limit [Å] | 3.700 |
Rmerge | 0.154 |
Number of reflections | 69231 |
<I/σ(I)> | 11.2 |
Completeness [%] | 99.8 |
Redundancy | 21.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM ammonium sulfate, 50 mM ADA pH 6.5, 9-12 % PEG4000 |