5LI4
bacteriophage phi812K1-420 tail sheath protein after contraction
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4.2 |
| Software | SPIDER,IHRSR,RELION,EPU,CTFFIND |
| CTF correction | PHASE FLIPPING ONLY |
