5LHX

PB3 Domain of Drosophila melanogaster PLK4 (Sak)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	35.870, 52.140, 42.530
Unit cell angles	90.00, 107.14, 90.00

Refinement procedure

Resolution	40.641 - 1.530
R-factor	0.1689
Rwork	0.167
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yyp
RMSD bond length	0.010
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.641
High resolution limit [Å]	1.530	1.530
Rmerge		0.891
Number of reflections	22170
<I/σ(I)>	11.3
Completeness [%]	97.8
Redundancy	3.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	150 nl protein solution (40.0 mg/ml protein in in 20 mM Tris pH 7.5, 150 mM NaCl, 2 mM DTT) and 50 nl mother liquor (1.5 M ammonium sulphate, 2% v/v PEG400, 100 mM Na HEPES pH 7.5)