5LHW
Central Coiled-Coil Domain of Human STIL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.800 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 40.310, 40.310, 29.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.663 - 0.910 |
R-factor | 0.1347 |
Rwork | 0.135 |
R-free | 0.13870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PolyAla helix based on 5AL6 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.607 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.663 | |
High resolution limit [Å] | 0.910 | 0.910 |
Rmerge | 0.668 | |
Number of reflections | 17643 | |
<I/σ(I)> | 24 | |
Completeness [%] | 99.1 | |
Redundancy | 11.2 | |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 292 | 150 nl protein solution (58.6 mg/ml protein in 20 mM Tris pH 7.5, 150 mM NaCl, 2 mM TCEP) and 150 nl of mother liquor (7 mM HEPES pH 8.2, 93 mM Tris pH 9.0, 55.36% v/v PEG 550 MME, 10% v/v glycerol) |