5LHV
X-ray structure of uridine phosphorylase from Vibrio cholerae in complex with uridine and sulfate ion at 1.29 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 64.223, 71.648, 88.878 |
Unit cell angles | 69.70, 72.70, 86.24 |
Refinement procedure
Resolution | 33.569 - 1.288 |
R-factor | 0.1454 |
Rwork | 0.145 |
R-free | 0.17830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k6o |
RMSD bond length | 0.014 |
RMSD bond angle | 1.633 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.230 | 46.230 | 1.370 |
High resolution limit [Å] | 1.288 | 3.850 | 1.288 |
Rmerge | 0.059 | 0.019 | 0.682 |
Number of reflections | 337058 | ||
<I/σ(I)> | 13.18 | 55.16 | 1.68 |
Completeness [%] | 94.0 | 98 | 89.8 |
Redundancy | 3.5 | 3.4 | |
CC(1/2) | 0.999 | 0.999 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG4000, 0.1M TRIS-HCl, 0.2M MgCl2x6H2O |