5LHR
The catalytic domain of murine urokinase-type plasminogen activator in complex with the active site binding inhibitory nanobody Nb22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.095, 66.506, 57.027 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 32.466 - 2.300 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.127 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.470 | |
| High resolution limit [Å] | 2.300 | |
| Rmerge | 0.107 | 0.407 |
| Number of reflections | 14379 | |
| <I/σ(I)> | 5.82 | |
| Completeness [%] | 94.8 | |
| Redundancy | 4 | |
| CC(1/2) | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | 0.2 M Ammonium Acetate, 0.1 M Tris, 16 % w/v PEG 10.000 |
