5LHK
Bottromycin maturation enzyme BotP in complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.89 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 151.510, 151.510, 101.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.210 - 2.320 |
| R-factor | 0.14891 |
| Rwork | 0.147 |
| R-free | 0.18977 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.210 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.142 | 0.736 |
| Number of reflections | 30149 | |
| <I/σ(I)> | 26.8 | 5.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 37.8 | 35.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 4000 (10 % w/v)), MES pH 6.5 (0.1 M) |
