5LGV
GlgE isoform 1 from Streptomyces coelicolor E423A mutant soaked in maltooctaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.920 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 113.611, 113.611, 313.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.920 - 2.500 |
R-factor | 0.1805 |
Rwork | 0.179 |
R-free | 0.20880 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4cn6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.431 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.6) |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.920 | 49.920 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.094 | 0.031 | 0.904 |
Number of reflections | 72101 | ||
<I/σ(I)> | 17.9 | 2.3 | |
Completeness [%] | 100.0 | 99 | 100 |
Redundancy | 13 | 13.5 | 8.2 |
CC(1/2) | 0.999 | 0.999 | 0.628 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | NULL |