5LGG

The N-terminal WD40 domain of Apc1 (Anaphase promoting complex subunit 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	82.633, 113.262, 100.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.131 - 2.150
R-factor	0.181
Rwork	0.179
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ui9
RMSD bond length	0.009
RMSD bond angle	1.198
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1810)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.131	2.223
High resolution limit [Å]	2.150	2.150
Rmerge	0.059	0.433
Number of reflections	25408
<I/σ(I)>	16.76	2.01
Completeness [%]	97.8	84.7
Redundancy	4.8	2.5
CC(1/2)	0.999	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	0.1 M citric acid, pH5.0, 2M NaCl