5LG6

Structure of the deglycosylated porcine aminopeptidase N ectodomain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	66.465, 215.690, 78.630
Unit cell angles	90.00, 91.90, 90.00

Refinement procedure

Resolution	24.982 - 2.500
R-factor	0.1918
Rwork	0.191
R-free	0.22940
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.266
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.100
Number of reflections	73869
<I/σ(I)>	6
Completeness [%]	97.0
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	5% PEG-1000, 10% PEG-8000