5LG4
Crystal structure of the Sec3/Sso2 complex at 2.9 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.123, 84.123, 237.001 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.828 - 2.900 |
R-factor | 0.2619 |
Rwork | 0.260 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3A58.pdb 1IFO.pdb |
RMSD bond length | 0.002 |
RMSD bond angle | 0.416 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.158 | 2.640 |
Number of reflections | 75872 | |
<I/σ(I)> | 15.3 | 1.2 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 6.6 | 6.4 |
CC(1/2) | 0.990 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | The small rod-like crystals were obtained in a condition containing 0.1 M Tris-HCl (pH 8.5) and 2 M (NH4)2SO4. |