5LFP

Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis (space group P6322, SeMet)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9785
Spacegroup name	P 63 2 2
Unit cell lengths	100.857, 100.857, 207.422
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.350 - 3.303
R-factor	0.2518
Rwork	0.254
R-free	0.31570
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.285
Data reduction software	XDS (May 1,2016)
Data scaling software	Aimless (0.5.26)
Phasing software	SHELX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.350	3.410
High resolution limit [Å]	3.300	3.300
Rmerge	0.110	1.001
Number of reflections	16086
<I/σ(I)>	10.4	1.6
Completeness [%]	90.7	87.9
Redundancy	9.5	7.68
CC(1/2)	0.999	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	5-7 % (v/v) propane-1,2-diol, 100 mM HEPES-NaOH pH 8.0 - 8.2, 200 mM MgCl2