5LFJ

Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	101.378, 101.378, 311.246
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.499 - 2.600
R-factor	0.2277
Rwork	0.224
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lfp
RMSD bond length	0.008
RMSD bond angle	1.167
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.500	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.070	0.723
Number of reflections	19401
<I/σ(I)>	17.83	2.96
Completeness [%]	100.0	100
Redundancy	9.7	9.6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	2-4 % (v/v) propane-2-ol, 100 mM HEPES-NaOH pH 7.5-8.2, 150-300 mM MgCl2