5LFD
Crystal structure of allantoin racemase from Pseudomonas fluorescens AllR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 109.720, 109.720, 109.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.830 - 2.150 |
| R-factor | 0.20528 |
| Rwork | 0.202 |
| R-free | 0.26414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eq5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.870 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.580 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.212 |
| Number of reflections | 24255 | |
| <I/σ(I)> | 19.5 | 12.1 |
| Completeness [%] | 97.4 | 86.1 |
| Redundancy | 9.4 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1M Tris pH 8.5, 8% w/v PEG 8000 |
