5LF8
Human Nucleoside diphosphate-linked moiety X motif 17 (NUDT17)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9900 |
Spacegroup name | H 3 2 |
Unit cell lengths | 151.913, 151.913, 81.495 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.960 - 2.560 |
R-factor | 0.23072 |
Rwork | 0.227 |
R-free | 0.29823 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.617 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.960 | |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.067 | |
Number of reflections | 11806 | |
<I/σ(I)> | 18 | |
Completeness [%] | 100.0 | |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M SPG pH 7.0 30% PEG1K |