5LEE

Crystal structure of DARPin-DARPin rigid fusion, variant DDD_D12_12_D12_12_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	69.260, 69.260, 230.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.279 - 2.401
R-factor	0.2506
Rwork	0.249
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.426
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.279	2.487
High resolution limit [Å]	2.401	2.401
Rmerge	0.092	0.230
Number of reflections	25927
<I/σ(I)>	18.38
Completeness [%]	96.7
Redundancy	19.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG 3350 20% w/v, Sodium citrate 0.2 M, Bis-Tris propane 0.1 M, pH 8.5