5LEC

Crystal structure of DARPin-DARPin rigid fusion, variant DDD_D12_12_D12_12_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	95.150, 104.590, 57.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.897 - 2.506
R-factor	0.2344
Rwork	0.233
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.440
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.897	2.596
High resolution limit [Å]	2.506	2.506
Rmerge	0.194	4.884
Number of reflections	20168
<I/σ(I)>	8.63
Completeness [%]	99.7
Redundancy	13
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	PEG 3350 20% w/v, Citric acid 0.04 M, Bis-Tris propane 0.06 M, pH 6.4