5LEB
Crystal structure of DARPin-DARPin rigid fusion, variant DDD_D12_06_D12_06_D12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-17 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.120, 29.470, 146.870 |
Unit cell angles | 90.00, 107.47, 90.00 |
Refinement procedure
Resolution | 35.343 - 2.300 |
R-factor | 0.2127 |
Rwork | 0.211 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1SVX chain A |
RMSD bond length | 0.002 |
RMSD bond angle | 0.425 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2398)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.343 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.073 | 1.931 |
Number of reflections | 24249 | |
<I/σ(I)> | 18.17 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 8000 10.0% w/v, PEG 1000 10.0% w/v, Sodium acetate 0.3 M, Sodium cacodylate 0.1 M, pH 6.5 |