5LEA

Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_12_3G124

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	62.410, 46.400, 63.080
Unit cell angles	90.00, 111.10, 90.00

Refinement procedure

Resolution	36.437 - 2.400
R-factor	0.2386
Rwork	0.236
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2398))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.437	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.094	1.381
Number of reflections	13295
<I/σ(I)>	9.4
Completeness [%]	99.0
Redundancy	4.2
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	MPD 32.3% v/v, Sodium Chloride 0.2 M, Sodium acetate trihydrate 0.1 M, pH 5.0