5LE9

Crystal structure of DARPin-DARPin rigid fusion, variant DD_Off7_09_3G124

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	71.980, 71.980, 128.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.859 - 1.850
R-factor	0.2267
Rwork	0.226
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.426
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.859	1.916
High resolution limit [Å]	1.850	1.850
Rmerge	0.178	18.360
Number of reflections	29498
<I/σ(I)>	13.26
Completeness [%]	99.4
Redundancy	24.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	MPD 30% v/v, Sodium chloride 0.2 M, Sodium acetate trihydrate 0.1 M, pH 4.6