5LE5
Native human 20S proteasome at 1.8 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.440, 202.770, 316.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 170.660 - 1.800 |
R-factor | 0.18358 |
Rwork | 0.182 |
R-free | 0.21175 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3une |
RMSD bond length | 0.016 |
RMSD bond angle | 1.778 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.1) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 170.660 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.091 | 2.910 |
Number of reflections | 633728 | |
<I/σ(I)> | 18.5 | 0.91 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 13.4 | 11 |
CC(1/2) | 1.000 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Bis-Tris, 0.2 M Magnesium Chloride, 10 % PEG3350 |