5LE3

Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_09_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	97.220, 108.790, 129.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.610 - 3.500
R-factor	0.3056
Rwork	0.303
R-free	0.34880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.002
RMSD bond angle	0.638
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2456: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	3.625
High resolution limit [Å]	3.500	3.500
Rmerge	0.241	12.980
Number of reflections	17776
<I/σ(I)>	7.96
Completeness [%]	98.5
Redundancy	12.9
CC(1/2)	0.999	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	293	PEG 3350 15.9% w/v, Sodium fluoride 0.2 M, Bis-Tris propane 0.1 M, pH 6.3