5LDR

Crystal structure of a cold-adapted dimeric beta-D-galactosidase from Paracoccus sp. 32d strain in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.017
Spacegroup name	P 21 21 21
Unit cell lengths	79.260, 107.580, 201.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.270 - 3.150
R-factor	0.18806
Rwork	0.184
R-free	0.26658
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5euv
RMSD bond length	0.017
RMSD bond angle	2.091
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.250
High resolution limit [Å]	3.150	3.150
Rmerge	0.176	0.848
Number of reflections	29579
<I/σ(I)>	7.77	1.63
Completeness [%]	96.7	94.4
Redundancy	3.65	3.64
CC(1/2)	0.983	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	24% PEG 2000 0.2M ammonium acetate 0.1M Bis-Tris pH 5.0