5LDA
Structure of deubiquitinating enzyme homolog (Pyrococcus furiosus JAMM1) in complex with ubiquitin-like SAMP2.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97939, 0.97947 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.110, 37.233, 59.528 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 46.730 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.641 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.557 | |
| Number of reflections | 16059 | |
| <I/σ(I)> | 14.84 | 1.99 |
| Completeness [%] | 98.0 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM Bis-Tris,200 mM NaCl, 25 % PEG3350, 10 % Glycerol |
