5LCZ
Chimeric GST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8081 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.920, 91.380, 51.490 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 19.669 - 2.325 |
| R-factor | 0.2036 |
| Rwork | 0.200 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pkw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.509 | |
| Number of reflections | 19517 | |
| <I/σ(I)> | 9.04 | 2.3 |
| Completeness [%] | 98.5 | 83.4 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 289 | PEG 4000 20% (w/v), ammonium citrate 0.2 M, pH 6.4 |
