5LCY
Formaldehyde-Responsive Regulator FrmR E64H variant from Salmonella enterica serovar Typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.790, 25.680, 100.500 |
Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
Resolution | 29.697 - 2.190 |
R-factor | 0.2478 |
Rwork | 0.244 |
R-free | 0.31150 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.010 |
RMSD bond angle | 1.126 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.697 | 2.250 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.495 | |
Total number of observations | 113762 | |
Number of reflections | 18039 | |
<I/σ(I)> | 12.2 | 3.2 |
Completeness [%] | 98.5 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20 mM NaCl, 23 % (w/v) poly(ethylene glycol) 4000, and 10 mM BisTris pH 6.5 |