5LCY
Formaldehyde-Responsive Regulator FrmR E64H variant from Salmonella enterica serovar Typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.790, 25.680, 100.500 |
| Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
| Resolution | 29.697 - 2.190 |
| R-factor | 0.2478 |
| Rwork | 0.244 |
| R-free | 0.31150 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.126 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.697 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.495 | |
| Total number of observations | 113762 | |
| Number of reflections | 18039 | |
| <I/σ(I)> | 12.2 | 3.2 |
| Completeness [%] | 98.5 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20 mM NaCl, 23 % (w/v) poly(ethylene glycol) 4000, and 10 mM BisTris pH 6.5 |
