5LCL
STRUCTURE OF the RAD14 DNA-binding domain IN COMPLEX WITH C8-aminofluorene- GUANINE CONTAINING DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-09 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 41 |
Unit cell lengths | 53.205, 53.205, 130.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.300 - 2.200 |
R-factor | 0.2184 |
Rwork | 0.217 |
R-free | 0.25555 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g32 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.029 |
Data reduction software | XDS (VERSION Oct 15, 2015) |
Data scaling software | XDS (VERSION Oct 15, 2015) |
Phasing software | REFMAC (5.8.0151) |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.168 | 0.900 |
Number of reflections | 18314 | |
<I/σ(I)> | 8.6 | 1.6 |
Completeness [%] | 99.0 | 93 |
Redundancy | 7 | 5.6 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 0.22M ammonium nitrate 38% 2-methyl-1,3,-propanediol |