5LCB
In situ atomic-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum obtained combining solid-state NMR spectroscopy, cryo electron microscopy and polarization spectroscopy
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE II | 700 | |
| 2 | Bruker | AVANCE II | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 13C-13C DARR |
| 2 | 1 | 1 | 3D NCACX |
| 3 | 1 | 1 | 3D NCOCX |
| 4 | 1 | 1 | 3D CANCO |
| 5 | 1 | 1 | 3D CONCA |
| 6 | 1 | 1 | 2D NCA |
| 7 | 1 | 1 | 2D NCO |
| 8 | 1 | 1 | 2D 13C-13C DARR |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7 | 1 | 280 |
Conformers
| Conformers Calculated Total Number | 80 |
| Conformers Submitted Total Number | 1 |
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 26.5 |
| Number of Particle | 1790 |
| Software | EMAN,Strul,SPIDER,EPU,CTFFIND |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
