5LCB
In situ atomic-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum obtained combining solid-state NMR spectroscopy, cryo electron microscopy and polarization spectroscopy
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
1 | Bruker | AVANCE II | 700 | |
2 | Bruker | AVANCE II | 500 |
Experiment
experiment id | conditions id | solution id | Experiment type |
1 | 1 | 1 | 2D 13C-13C DARR |
2 | 1 | 1 | 3D NCACX |
3 | 1 | 1 | 3D NCOCX |
4 | 1 | 1 | 3D CANCO |
5 | 1 | 1 | 3D CONCA |
6 | 1 | 1 | 2D NCA |
7 | 1 | 1 | 2D NCO |
8 | 1 | 1 | 2D 13C-13C DARR |
NMR Sample
conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
1 | 7 | 1 | 280 |
Conformers
Conformers Calculated Total Number | 80 |
Conformers Submitted Total Number | 1 |
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK II |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 26.5 |
Number of Particle | 1790 |
Software | EMAN,Strul,SPIDER,EPU,CTFFIND |
Symmetry | C1 |
CTF correction | PHASE FLIPPING ONLY |