5LC3
Xray structure of mouse FAM3C ILEI monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.839, 31.976, 75.748 |
| Unit cell angles | 90.00, 111.55, 90.00 |
Refinement procedure
| Resolution | 44.250 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yop |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.300 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.042 |
| Number of reflections | 23649 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 98.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30 mg/mL + 17.5% w/v PEG4000, 10% glycerol and 82mM (NH4)2SO4 |
