5LC2
Xray structure of human FAM3C ILEI monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 41 |
| Unit cell lengths | 110.650, 110.650, 30.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yop |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.100 |
| High resolution limit [Å] | 1.590 |
| Rmerge | 0.058 |
| Number of reflections | 46062 |
| <I/σ(I)> | 14.4 |
| Completeness [%] | 91.7 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25% PEG monomer ether 550, 100 mM MES pH 6.5, 10mM ZnSO4 |
