5LBR
Wild-type PAS-GAF fragment from Deinococcus radiodurans Bphp collected at SACLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2015-02-01 |
| Detector | MPCCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.220, 55.490, 71.630 |
| Unit cell angles | 90.00, 92.84, 90.00 |
Refinement procedure
| Resolution | 71.540 - 2.200 |
| R-factor | 0.18084 |
| Rwork | 0.182 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q0h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.287 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.540 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 18818 | |
| <I/σ(I)> | 5.62 | 0.68 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 81.2 | 24.1 |
| CC(1/2) | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.95 | 298 | Sodium Acetate, Peg400, 2-methyl-2,4-pentanediol |
