5LBI
Apo-structure of humanised RadA-mutant humRadA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9799 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.350, 77.980, 39.810 |
| Unit cell angles | 90.00, 117.53, 90.00 |
Refinement procedure
| Resolution | 35.300 - 1.430 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.520 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.052 | 0.257 |
| Number of reflections | 36018 | |
| <I/σ(I)> | 20.51 | 5.56 |
| Completeness [%] | 97.1 | 91.4 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 4-8% PEG1000, 100 mM Na/KPO4, pH 5.6 |
