5LBH
Crystal structure of Helicobacter cinaedi CAIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.746, 135.918, 93.445 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 46.533 - 2.553 |
| R-factor | 0.1902 |
| Rwork | 0.187 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.530 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.149 | 0.859 |
| Number of reflections | 66294 | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 99.2 | 61.9 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 0.1 M SPG (pH 4.0) and 25% w/v poly(ethylene glycol) 1500 as a precipitant (solution number 1 PACT Premier HT-96 kit; Molecular Dimensions, Suffolk, UK) |
