5LAF
Room temperature X-ray diffraction of tetragonal HEWL with 1M of uridine. First data set (0.31 MGy)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9840019 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.110, 79.110, 37.897 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.560 - 1.500 |
R-factor | 0.14572 |
Rwork | 0.144 |
R-free | 0.17636 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1bwh |
RMSD bond length | 0.025 |
RMSD bond angle | 2.329 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.560 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.312 |
Number of reflections | 19787 | |
<I/σ(I)> | 26.6 | 5.6 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.4 | 6.4 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 291 | protein: 50 mg/ml of HEWL in mQ crystallization solution: 200mM NaAcetate pH 4.7 + 10% NaCl |