5LAD
Crystal Structure of apo HydF from thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 4 |
| Unit cell lengths | 126.140, 126.140, 68.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 3.000 |
| R-factor | 0.248 |
| Rwork | 0.244 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS (VERSION Oct 15, 2015) |
| Data scaling software | XDS (VERSION Oct 15, 2015) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | |
| High resolution limit [Å] | 3.000 | |
| Rmeas | 0.051 | 0.157 |
| Number of reflections | 10940 | |
| <I/σ(I)> | 17.48 | |
| Completeness [%] | 97.4 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5 | 293 | 0.3 M sodium malonate dibasic monohydrate, 0.1M sodium acetate pH 5.0, 8% w/v gamma poly glutamic acid Anaerobic glovebox <1 ppm O2 |
