5LA6
Tubulin-pironetin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.680, 157.200, 180.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.270 - 2.100 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4i4t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 175571 | |
| <I/σ(I)> | 18.9 | 0.6 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 13.6 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 293 | 5% PEG, 12% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole |
