5LA1
The mechanism by which arabinoxylanases can recognise highly decorated xylans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9772 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.911, 72.514, 109.537 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.160 - 1.900 |
R-factor | 0.13097 |
Rwork | 0.129 |
R-free | 0.16041 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5AK1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.621 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.160 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.028 | 0.084 |
Number of reflections | 42716 | |
<I/σ(I)> | 26.6 | 8 |
Completeness [%] | 98.6 | 85 |
Redundancy | 3.6 | 2.1 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | 100 mM Na/K phosphate, 100 mM MES pH 6.5 and 2M NaCl. |