5L9S
Structure of Agrobacterium tumefaciens C58 strain PBP AttC in open unliganded conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.010, 118.450, 121.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.590 - 2.490 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l9g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.490 | 2.490 |
Number of reflections | 24641 | |
<I/σ(I)> | 11.57 | |
Completeness [%] | 99.0 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 40% PEG 4K, 0.1M MES pH 6.5, 0.2M ammonium sulfate |