5L9N
Structure of uridylylated GlnB from Escherichia coli bound to ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97917 |
Spacegroup name | I 21 3 |
Unit cell lengths | 88.868, 88.868, 88.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.103 - 1.901 |
R-factor | 0.1855 |
Rwork | 0.184 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pil |
RMSD bond length | 0.008 |
RMSD bond angle | 1.243 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.400 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.064 | 0.618 |
Number of reflections | 9346 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 99.7 | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0,1 M NaAcetate pH 4.6, 20 mM CaCl2, 30% MPD |