5L9D
AFAMIN ANTIBODY FRAGMENT, N14 FAB, L1- GLYCOSYLATED, CRYSTAL FORM I, parsimonious model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.782, 69.249, 87.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.380 - 1.880 |
| R-factor | 0.17819 |
| Rwork | 0.176 |
| R-free | 0.21505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1il1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.355 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.370 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.076 | 0.800 |
| Number of reflections | 34249 | |
| <I/σ(I)> | 99.8 | 1.4 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 4.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 291 | 200NL FAB 10MG/ML IN 20 MM HEPES PH 7.5 REMARK 280 7.5, 150 MM NACL PLUS 200NL 30%W/V PEG 1K, 200MM KF, pH 5.8) |
