5L9C
Crystal structure of an endoglucanase from Penicillium verruculosum in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.330, 73.230, 91.150 |
| Unit cell angles | 87.23, 94.17, 100.52 |
Refinement procedure
| Resolution | 48.398 - 1.850 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i6s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.659 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.102 | 0.498 |
| Number of reflections | 107169 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 90.0 | |
| Redundancy | 1.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | 20% polyethylene glycole 6000, 50 mM bis-tris propane pH 7.0, 7% 2 methyl-2,4,-pentanediol, 5% 2 methyl-2 propanal, 6% PEG 5000, cellobiose |
