5L9C
Crystal structure of an endoglucanase from Penicillium verruculosum in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2014-04-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 |
Unit cell lengths | 49.330, 73.230, 91.150 |
Unit cell angles | 87.23, 94.17, 100.52 |
Refinement procedure
Resolution | 48.398 - 1.850 |
R-factor | 0.2288 |
Rwork | 0.226 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i6s |
RMSD bond length | 0.006 |
RMSD bond angle | 0.659 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 1.830 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.102 | 0.498 |
Number of reflections | 107169 | |
<I/σ(I)> | 6.1 | |
Completeness [%] | 90.0 | |
Redundancy | 1.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 285 | 20% polyethylene glycole 6000, 50 mM bis-tris propane pH 7.0, 7% 2 methyl-2,4,-pentanediol, 5% 2 methyl-2 propanal, 6% PEG 5000, cellobiose |