5L9A
L-threonine dehydrogenase from trypanosoma brucei.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8726 |
Spacegroup name | P 1 |
Unit cell lengths | 46.985, 57.722, 70.074 |
Unit cell angles | 72.46, 70.38, 73.20 |
Refinement procedure
Resolution | 46.820 - 1.450 |
R-factor | 0.10254 |
Rwork | 0.100 |
R-free | 0.15643 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yy7 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.070 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.820 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.089 | 0.398 |
Number of reflections | 109054 | |
<I/σ(I)> | 10.4 | 2.7 |
Completeness [%] | 95.0 | 82.1 |
Redundancy | 3.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 20 % w/v PEG 10K, TDH 2.0 mg/ml |