5L93
An atomic model of HIV-1 CA-SP1 reveals structures regulating assembly and maturation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 3.9 |
| Software | CCP4 package,PHENIX,Coot,AV3,TOM Toolbox,SerialEM,CTFFIND,CTFPHASEFLIP,UCSF Chimera |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING ONLY |
